Data from: Exome genotyping, linkage disequilibrium and population structure in loblolly pine (Pinus taeda L.)

Loblolly pine (Pinus taeda L.) is one of the most widely planted and commercially important forest tree species in the USA and worldwide, and is an object of intense genomic research. However, whole genome resequencing in loblolly pine is hampered by its large size and complexity and a lack of a good reference. As a valid and more feasible alternative, entire exome sequencing was hence employed to identify the gene-associated single nucleotide polymorphisms (SNPs) and to genotype the sampled trees.

Genomics and Genetics

Data from: Chromosome-level genome assembly and transcriptome of the green alga Chromochloris zofingiensis illuminates astaxanthin production

For genome assembly of C. zofingiensis strain SAG 211–14, we used a hybrid approach blending short reads (Illumina), long reads (Pacific Biosciences of California), and whole-genome optical mapping (OpGen) (SI Appendix, SI Text and Datasets S1–S19, and refer to SI Appendix, Datasets Key). The combined power of these approaches yielded a high-quality haploid nuclear genome of C. zofingiensis of ∼58 Mbp distributed over 19 chromosomes (Fig. 2) in the tradition of model organism projects, as opposed to the fragmentary “gene-space” assemblies typical of modern projects using high-throughput methods and associated software. Approximately 99% of reads from the Illumina genomic libraries were accounted for, and nonplaceholder chromosomal sequence covers ∼94% of the optical map. Because no automated pipeline was found able to achieve the desired quality, methods are described in SI Appendix, SI Text.

Genomics and Genetics

Genes of viral origin in the Microplitis demolitor genome

Microplitis demolitor (Hymenoptera: Braconidae) is a parasitoid used as a biological control agent to control larval-stage Lepidoptera and serves as a model for studying the function and evolution of symbiotic viruses in the genus Bracovirus. Using RNA-Seq data for this species and manual annotation of genes of viral origin, we annotated a high-quality gene set including 171 virus-derived protein-coding genes.

insects 5000 program

Professor: A Motorized Field-Based Phenotyping Cart

An easy-to-customize, low-cost, low disturbance, motorized, and adjustable proximal sensing cart for field-based high-throughput phenotyping is described. General dimensions, motor specifications, and a remote operation application are given. The cart, named "Professor", supports mounting multiple proximal sensors and cameras for characterizing plant traits grown under field conditions.

Professor field phenotyping cart with image array in a cotton field.

The GRIN-Global Project

GRIN-Global (GG) is a database application that enables genebanks to store and manage information associated with plant genetic resources (germplasm) and deliver that information globally. The GRIN-Global project's mission is to provide a scalable version of the Germplasm Resource Information Network (GRIN) suitable for use by any interested genebank in the world.

Ag Data Commons

National Microbial Germplasm Program

The goal of the National Microbial Germplasm Program is to ensure that the genetic diversity of agriculturally important microorganisms is maintained to enhance and increase agricultural efficiency and profitability. The program collects, authenticates, and characterizes potentially useful microbial germplasm; preserves microbial genetic diversity; and facilitates distribution and utilization of microbial germplasm for research and industry.

Agroecosystems & Environment