Data from: Proteomics analysis reveals novel host molecular mechanisms associated with thermotherapy of 'Ca. Liberibacter asiaticus'-infected citrus plants

The goal of this study is to identify potential Las resistance/tolerance-related genes in citrus plants for application in breeding or genetic engineering programs, and apply comparative proteomics analysis via 2-DE and mass spectrometry to elucidate the molecular processes associated with heat-induced mitigation of HLB in citrus plants.

Genomics and Genetics

Data from: Genetic Architecture of Resistance to Stripe Rust in a Global Winter Wheat Germplasm Collection

Virulence shifts in populations of Puccinia striiformis f. sp. tritici (Pst), the causal pathogen of wheat stripe rust, are a major challenge to resistance breeding. The majority of known resistance genes are already ineffective against current races of Pst, necessitating the identification and introgression of new sources of resistance. Germplasm core collections that reflect the range of genetic and phenotypic diversity of crop species are ideal platforms for examining the genetic architecture of complex traits such as resistance to stripe rust. We report the results of genetic characterization and genome-wide association analysis (GWAS) for resistance to stripe rust in a core subset of 1175 accessions in the National Small Grains Collection (NSGC) winter wheat germplasm collection, based on genotyping with the wheat 9K single nucleotide polymorphism (SNP) iSelect assay and phenotyping of seedling and adult plants under natural disease epidemics in four environments.

Genomics and Genetics

Data from: Condition‐dependent co‐regulation of genomic clusters of virulence factors in the grapevine trunk pathogen Neofusicoccum parvum

The capability of the ascomycete Neofusicoccum parvum to colonize woody tissue, combined with the secretion of phytotoxic compounds, is thought to underlie its pathogenicity and virulence. The repertoire of virulence factors and their transcriptional dynamics as the fungus feeds on different substrates and colonizes the woody stem are described and a highly contiguous genome is assembled and annotated using single‐molecule real‐time DNA sequencing.

Genomics and Genetics

Data from: Agro-environmental consequences of shifting from nitrogen- to phosphorus-based manure management of corn.

This experiment was designed to measure greenhouse gas (GHG) fluxes and related agronomic characteristics of a long-term corn-alfalfa rotational cropping system fertilized with manure (liquid versus semi-composted separated solids) from dairy animals. Different manure-application treatments were sized to fulfill two conditions: (1) an application rate to meet the agronomic soil nitrogen requirement of corn (“N-based” without manure incorporation, more manure), and (2) an application rate to match or to replace the phosphorus removal by silage corn from soils (“P-based” with incorporation, less manure). In addition, treatments tested the effects of liquid vs. composted-solid manure, and the effects of chemical nitrogen fertilizer. The controls consisted of non-manured inorganic N treatments (sidedress applications). These activities were performed during the 2014 and 2015 growing seasons as part of the Dairy Coordinated Agricultural Project, or Dairy CAP, as described below. The data from this experiment give insight into the factors controlling GHG emissions from similar cropping systems, and may be used for model calibration and validation after careful evaluation of the flagged data.

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