i5k Workspace

About the i5k Workspace@NAL

The i5k Workspace (https://i5k.nal.usda.gov) is an inclusive genome portal for any arthropod genome project that would like to make use of our resources. We provide download services, BLAST, the JBrowse genome browser, and the Apollo manual curation service. Over 50 arthropod genomes are now part of the i5k Workspace, and users are encouraged to browse the genomes that we host, and contribute to the curation of each genome. For more information about the i5k Workspace, you can read our paper on the i5k Workspace, view our posters and talks, and find our software projects on github. The Ag Data Commons is now hosting a growing number of i5k Workspace datasets.

About the i5k initiative

The i5k initiative is a transformative project that aims to sequence and analyze the genomes of 5,000 arthropod species. The National Agricultural Library has partnered with the i5k initiative to create the i5k Workspace@NAL, which serves any ‘orphaned’ arthropod genome project's hosting needs. For more information about the i5k initiative, read the paper and visit the website.

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i5k Datasets

46 datasets

Hyalella azteca Official Gene Set v1.0

The Hyalella azteca genome was recently sequenced and annotated as part of the i5k pilot project by the Baylor College of Medicine. The Hyalella azteca research community has manually reviewed and curated the computational gene predictions and generated an official gene set, OGSv1.0. The OGS is an integration of automatic gene predictions from Maker with manual annotations by the research community (via the Apollo manual annotation software).

insects 5000 program

Trichogramma pretiosum genome annotations v0.5.3

This dataset presents the Trichogramma pretiosum gene set BCM_v_0.5.3. RNA-Seq data was used with additional protein homology data for a MAKER automated annotation of the Trichogramma pretiosum genome assembly 1.0.

insects 5000 program

Trichogramma pretiosum genome assembly v1.0

The Baylor College of Medicine has sequenced and annotated the Trichogramma pretiosum genome as part of the i5k pilot project. This dataset presents the Trichogramma pretiosum genome v1.0. This assembly version is the pre-release version, prior to filtering and quality control by the National Center for Biotechnology Information's GenBank resource. Scaffold 109 was identified to be a complete Wolbachia genome, and was removed from the assembly on GenBank and relocated to a separate record (https://www.ncbi.nlm.nih.gov/nuccore/NZ_CM003641.1).

insects 5000 program

Diaphorina citri Official Gene Set v1.0

This gene set (OGS v1.0) combines both automatically predicted and manually curated gene models. This community effort produced 530 manually curated gene models across developmental, physiological, RNAi regulatory, and immunity-related pathways. As previously shown in the pea aphid, RNAi machinery genes putatively involved in the microRNA pathway have been specifically duplicated. A comprehensive transcriptome enabled us to identify a number of gene families that are either missing or misassembled in the draft genome.

Asian citrus psyllid (Diaphorina citri)

Data from: Integrating classical genetics with chromosome-scale genome assembly to characterize a genetic sexing system in Bactrocera cucurbitae (Diptera: Tephritidae)

This data supports the manuscript "Integrating classical genetics with chromosome-scale genome assembly to characterize a genetic sexing system in Bactrocera cucurbitae (Diptera: Tephritidae)". The melon fly, Bactrocera cucurbitae, is a destructive agricultural pest and is the subject of strict quarantines that are enforced to prevent its establishment outside of its current geographic range. In addition to quarantine efforts, additional control measures are necessary for its eradication in the case of invasion to agriculturally rich areas. The sterile insect technique (SIT) has been effective in the control of several invertebrate pest species, and is part of a management strategy that regulatory agencies would like to expand to other important pests such as the melon fly.

insects 5000 program

Onthophagus taurus Genome Annotations v0.5.3

The Baylor College of Medicine recently sequenced and annotated the Onthophagus taurus genome as part of the i5k pilot project. NOTE: This gene set is an unstable pre-release (v0.5.3), and was provided to facilitate manual curation and analyses before the official gene set is released. Gene identifiers from this gene set will likely not be maintained.

Male adult Onthophagus taurus dung beetle

Athalia rosae genome annotations v0.5.3

This dataset presents the Athalia rosae gene set BCM_v_0.5.3. RNA-Seq data was used with additional protein homology data for a MAKER automated annotation of the Athalia rosae genome assembly 1.0.

insects 5000 program

Athalia rosae Official Gene Set OGSv1.0

The Athalia rosae genome was recently sequenced and annotated as part of the i5k pilot project by the Baylor College of Medicine. The Athalia rosae research community has manually reviewed and curated the computational gene predictions and generated an official gene set, OGSv1.0. The general procedure for generating this OGS is outlined here: https://github.com/NAL-i5K/I5KNAL_OGS/wiki. OGSv1.0 was generated by merging gene set AROS-V0.5.3-Models generated by the Baylor College of Medicine, and community-curated models in the Apollo software, after QC of the Apollo output.

insects 5000 program