The Aquatic eDNAtlas Project: Lab Results Map - USFS RMRS

The eDNA samples in the eDNAtlas database describe species occurrence locations and were collected by the U.S. Forest Service and numerous agencies that have partnered with the National Genomics Center for Wildlife and Fish Conservation (NGC) throughout the United States. The eDNAtlas is accessed via an interactive ArcGIS Online (AGOL) map that allows users to view and download sample site information and lab results of species occurrence for the U.S. The results are primarily based on samples analyzed at the National Genomics Center for Wildlife and Fish Conservation (NGC) and associated with geospatial attributes created by the Boise Spatial Streams Group (BSSG).

Genomics and Genetics

The Range-Wide Bull Trout eDNA Project - USFS RMRS

The bull trout (Salvelinus confluentus) eDNA survey results Online Map allows users to view the survey results in an interactive map by coupling 1) predictions from the range-wide, spatially precise Climate Shield model on the location of natal habitats of bull trout with 2) a sampling template for every 8-digit hydrologic unit in the historical range of bull trout, based on the probability of detecting bull trout presence using environmental DNA (eDNA) sampling. The map provides the ability to zoom in and look at an area of interest, as well as to create queries or select an area to download points as a shapefile.

Genomics and Genetics

Divergence in host specificity and genetics among populations of Aphelinus certus

These are data on variation in host specificity and genetics among 16 populations of an aphid parasitoid, Aphelinus certus, 15 from Asia and one from North America. Host range was the same for all the parasitoid populations, but levels of parasitism varied among aphid species, suggesting adaptation to locally abundant aphids. Differences in host specificity did not correlate with geographical distances among parasitoid populations, suggesting that local adaption is mosaic rather than clinal, with a spatial scale of less than 50 kilometers. Analysis of reduced representation libraries for each population showed genetic differentiation among them. Differences in host specificity correlated with genetic distances among the parasitoid populations.

Genomics and Genetics

Annotations of Unigenes Assembled from Schizaphis graminum and Sipha flava

Transcriptomes were assembled de novo from pools of adult aphids that were feeding on sorghum and switchgrass. Reads from all replicates were pooled, normalized in silico to 25X coverage, and assembled using Trinity. Only the most abundant isoform for each unigene was retained for annotation and unigenes with transcripts per million mapped reads (TPM) less than 0.5 were removed from the dataset. The remaining unigenes were annotated using Trinotate with BLASTP comparisons against the Swiss-Prot/UniProt database. In addition, Pfam-A assignments were computed using hmmer, signal peptide predictions were performed using SignalP, and transmembrane domain predictions were performed using tmHMM. Gene ontology (GO assignments) were retrieved from Trinotate using the highest scoring BLASTp matches as queries.

Genomics and Genetics

Data from: Characterization of Adult Transcriptomes from the Omnivorous Lady Beetle Coleomegilla maculata Fed Pollen or Insect Egg Diet

Expressed genes from two individual sibling specimens of Coleomegilla maculata (Coleoptera: Coccinellidae). One individual was fed only insect eggs as an adult, and one was fed only pollen as an adult. Two sequenced samples, total RNA from a single individual adult specimen of Coleomegilla maculata, a beneficial lady beetle common in agroecosystems and native to North America. One sample was an adult fed only insect eggs (carnivore diet) and one sample was an adult fed only pollen (plant-based diet); insects were reared from the same egg mass (siblings), fed identical diet while in larval stage.

Data from: The assembled transcriptome of the adult horn fly, Haematobia irritans

To better understand the adult horn fly, Haematobia irritans irritans, and the development of resistance in natural populations, an Illumina paired-end read HiSeq and GAII approach was used to determine the transcriptomes of untreated control adult females, untreated control adult males, permethrin-treated surviving adult males and permethrin + piperonyl butoxide-treated killed adult males from a Louisiana population of horn flies with a moderate level of pyrethroid resistance.

Genomics and Genetics

Arctic Peregrine Falcon Abundance on Cliffs Along the Colville River, Alaska, 1981-2002 and Covariate Input Files

This data set consists of fourteen data files. Rcode_arctic_peregrine_abundance.R contains R code that was used to analyze Arctic peregrine falcon data collected between 1981 and 2002. The code primarily uses the R package "UNMARKED" and is based on the Dail-Madsen model for estimating population abundance. To run this code in an R environment, download the file and open it in an R interpreter (such as RStudio). The remaining files are all covariate matrices that act as inputs to the R code.

Arctic Peregrine Falcon

ELIGULUM-A regulates lateral branch and leaf development. Original figure files

TIFF and JPEG files for the photographs used in constructing figures and supplemental figures in the manuscript, "ELIGULUM-A regulates lateral branch and leaf development," submitted to Plant Physiology. The images document a mutation that alters most of the structures of the plant and how the ELIGULUM-A gene interacts with different developmental pathways. The Figure Legend files describe the images individually.

branch and leaf development illustration