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Data from: Starch and dextrose at 2 levels of rumen-degradable protein in iso-nitrogenous diets: Effects on lactation performance, ruminal measurements, methane emission, digestibility, and nitrogen balance of dairy cows.

    This feeding trial was designed to investigate two separate questions. The first question is, “What are the effects of substituting two non-fiber carbohydrate (NFC) sources at two rumen-degradable protein (RDP) levels in the diet on apparent total-tract nutrient digestibility, manure production and nitrogen (N) excretion in dairy cows?”. This is relevant because most of the N ingested by dairy cows is excreted, resulting in negative effects on environmental quality. The second question is, “Is phenotypic residual feed intake (pRFI) correlated with feed efficiency, N use efficiency, and metabolic energy losses (via urinary N and enteric CH4) in dairy cows?”. The pRFI is the difference between what an animal is expected to eat, given its level of productivity, and what it actually eats. The goal was to determine whether production of CH4, urinary N or fecal N is a driver of pRFI.

    Vaccination Against Lawsonia intracellularis Decreases Shedding of Salmonella enterica serovar Typhimurium in Co-Infected Pigs and Alters the Gut Microbiome

      *Salmonella enterica* is a leading cause of foodborne illness worldwide and pork can serve a source of infection. In this study, we investigated if vaccinating pigs against L*awsonia intracellularis*, a common pathogen of swine that has previously been shown to favor *Salmonella enterica* infection, confers protection against *Salmonella enterica serovar Typhimurium*. We investigated the underlying changes in the gut microbiome mediated by single *S. Typhiumurium* infection compared to co-infection with *L. intracellularis* as well as the effect of vaccination on the microbiome.

      Low-Disturbance Manure Incorporation

        The LDMI experiment (Low-Disturbance Manure Incorporation) was designed to evaluate nutrient losses with conventional and improved liquid dairy manure management practices in a corn silage (*Zea mays*) / rye cover-crop (*Secale cereale*) system. The improved manure management treatments were designed to incorporate manure while maintaining crop residue for erosion control. Field observations included greenhouse gas (GHG) fluxes from soil, soil nutrient concentrations, crop growth and harvest biomass and nutrient content, as well as monitoring of soil physical and chemical properties. Observations from LDMI have been used for parameterization and validation of computer simulation models of GHG emissions from dairy farms (Gaillard et al., submitted). The LDMI experiment was performed as part of the Dairy CAP.

        Manure application methods for alfalfa-grass

          The MAMA experiment (Manure Application Methods for Alfalfa-Grass), from the USDA-ARS research station in Marshfield, WI was designed to evaluate nutrient and pathogen losses with conventional and improved liquid dairy manure management practices for alfalfa-grass production. Observations from MAMA have also been used for parameterization and validation of computer simulation models of greenhouse gas (GHG) emissions from dairy farms.