This dataset was generated from soybean (*Glycine max*) field trials conducted at the West Tennessee Research and Education Center in Jackson, TN and at the Research and Education Center at Milan in Milan, TN as well as from molecular marker screening conducted at the West Tennessee Research and Education Center in Jackson, TN. Table 3 includes measured data for height, yield, and seed size, and rating data for lodging and seed quality for JTN-5110, 5601T, and select other released germplasm lines and cultivars. Table 5 includes rating data for JTN-5110 and soybeans with established SCN resistance from simple sequence repeat (SSR) markers: Satt309 and Sat_168, associated with *rhg1* on chromosome 18; Sat_162, associated with *Rhg4* on chromosome 8; and Satt574, associated with *cqSCN-005* on chromosome 17. Table 7 includes rating data for JTN-5110 and check cultivars from frogeye leafspot (caused by *Cercospora sojina*) field disease screenings conducted in Milan, TN from 2010-2012.