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Data from: Fitness and host use remain stable in biological control agent after many years of hybridization

    This data was generated to test how hybridization of an introduced insect biological control agent impacts host use. The data characterize the species composition of individual *Diorhabda spp.* collected across New Mexico and Texas, USA. Species composition of each individual was determined using genomic methods and Structure. Lab reared colonies were tested for host specificity, by measuring feeding preference in 24-hour feeding tests and measuring frass deposited below three host plants. Data on several attributes were also collected, including body mass, fecundity during the 24-hour feeding trial, and oviposition preference. The larval offspring of these individuals were also reared in families for 12 days on the three host plants and larval survival and mass were measured to quantify larval performance on the hosts.


      OTUshuff is a set of tools written in Perl to identify and correct for the presence of pseudo-diversity in contrasts between microbial communities. The variably weighted Odum dissimilarity (DwOdum) allows for down-weighting the influence of either abundant or rare taxa in calculating a measure of similarity between two communities. We show that down-weighting the influence of rare taxa can be used to minimize pseudo b-diversity arising from incomplete sampling. Down-weighting the influence of abundant taxa can increase the sensitivity of hypothesis testing. OTUshuff is an associated test for identifying the presence of pseudo-diversity in pairwise community contrasts.


        myPhyloDB is an open-source software package aimed at developing a user-friendly web-interface for accessing and analyzing all of your laboratory's microbial ecology data (currently supported project types: soil, air, water, microbial, and human-associated). The storage and handling capabilities of myPhyloDB archives users' raw sequencing files, and allows for easy selection of any combination of projects/samples from all of your projects using the built-in SQL database. The data processing capabilities of myPhyloDB are also flexible enough to allow the upload, storage, and analysis of pre-processed data or raw (454 or Illumina) data files using the built-in versions of Mothur and R.