Data from: A High-Quality Genome Assembly from a Single, Field-collected Spotted Lanternfly (Lycorma delicatula) using the PacBio Sequel II System

A 2.3 Gb de novo genome assembly of a field-collected adult female Spotted Lanternfly (Lycorma delicatula) using a single PacBio SMRT Cell is provided. Supporting files for the manuscript "A High-Quality Genome Assembly from a Single, Field-collected Spotted Lanternfly (Lycorma delicatula) using the PacBio Sequel II System", include several intermediate versions of the assembly (raw output from Falcon, raw output from Falcon unzip, etc.) as well as the final assembly primary contigs and haplotigs (for the regions of the genome that were phased).

SLF-spotted lanternfly (Lycorma delicatula); adult winged. Photo: Stephen Ausmus, ARS Image Gallery

Data from: Metabolic Coevolution in the Bacterial Symbiosis of Whiteflies and Related Plant Sap-Feeding Insects

Genomic decay is a common feature of intracellular bacteria that have entered into symbiosis with plant sap-feeding insects. This study of the whitefly Bemisia tabaci and two bacteria (Portiera aleyrodidarum and Hamiltonella defensa) cohoused in each host cell investigated whether the decay of Portiera metabolism genes is complemented by host and Hamiltonella genes, and compared the metabolic traits of the whitefly symbiosis with other sap-feeding insects (aphids, psyllids, and mealybugs).

Genomics and Genetics

USDA ARS National Rhizobium Germplasm Collection

Our mission is to support application of low-input sustainable agriculture by: Providing, to the best of our ability, technical information about rhizobia, their preservation, and cultural and symbiotic characteristics; Acquiring and preserving the nitrogen-fixing bacterial symbionts of leguminous plants with the goal of maintaining widest possible genetic diversity; Maintaining quality control of new and existing germplasm by evaluation of microbiological purity and by examination of nodulation of the original trap host plant; Distributing cultures to the public and private sectors without charge for these services; Developing or adapting techniques in molecular biology for the determination of genetic diversity of rhizobia, to investigate interactions with their host plants and to identify novel characteristics; Acquiring, maintaining, evaluating quality, and distributing type strains for all the different taxa of nitrogen-fixing legume symbionts; Participating in the UNESCO program.

Genomics and Genetics

Data from: Defensive aphid symbiont Hamiltonella defensa effects on Aphelinus glycinis and Aphelinus atriplicis

Aphelinus glycinis was collected in the Peoples Republic of China under a Memorandum of Understanding between their Ministry of Agriculture and the United States Department of Agriculture (USDA). Aphelinus atriplicis was collected by employees of the USDA, Agricultural Research Service (ARS), in the Republic of Georgia with the permission of that government. The parasitoids were imported into the USDA, ARS, Beneficial Insect Introductions Research Unit containment facility in Newark, Delaware, under permits from the USDA, Animal and Plant Health Inspection Service (Permit Numbers P526P-08-02142 and P526P-09-01929). No specific permissions were required to collect Aphis craccivora or Acyrthosiphon pisum because these are cosmopolitan aphids that occur in the field throughout North America. None of the species collected or studied are endangered or protected.

Agroecosystems & Environment