Watermelon (Citrullus lanatus) and other cucurbits are important crops grown in Guatemala for local consumption and export. Four samplings of crowns, peduncles, and/or leaves of symptomatic plants were made in March and April 2015. Total RNA was extracted from symptomatic plant tissue and tested by RT-PCR for SqVYV, CYSDV, PRSV, and/or begomoviruses. This is the first report of SqVYV infecting watermelon in Central America.
To study the impact of wheat streak mosaic virus on global gene expression in wheat curl mite, we generated a de novo transcriptome assembly using 50 x 50 paired end reads from the Illumina HiSeq 2500. Reads were assembled using Trinity (version 2.0.6) and contigs greater than 200 nt were retained. All assembled transcripts were annotated using the Trinotate pipeline using blastp searches against the Swiss-prot/Uni-Prot database, blastx searches against the Swiss-prot/Uni-Prot databases, HMM searches against the Pfam-A database, blastp searches against the non-redundant protein database, and signalP and tmHMM predictions. To reduce noise from low abundance transcripts not well supported by the data, we filtered the assembly to retain only those transcripts with TPM values >=0.5.
The National Rabies Management Program conducts ORV operations in many US states. State summaries, maps, and statistics for oral rabies vaccine distribution can be accessed through this database.
Microplitis demolitor (Hymenoptera: Braconidae) is a parasitoid used as a biological control agent to control larval-stage Lepidoptera and serves as a model for studying the function and evolution of symbiotic viruses in the genus Bracovirus. Using RNA-Seq data for this species and manual annotation of genes of viral origin, we annotated a high-quality gene set including 171 virus-derived protein-coding genes.
Fopius arisanus (Sonan) is a braconid wasp (subfamily Opiinae) and biological control agent of a broad range of tephritid fruit fly species, including the global pests Mediterranean fruit fly Ceratitis capitata and the Oriental fruit fly Bactrocera dorsalis. In an effort to create foundational genomic resources for this species, the complete genome and transcriptomes for several wasp life stages have been recently generated. Manual annotation of 55 viral genes and phylogenetic analysis revealed that F. arisanus has independently acquired a symbiotic virus related to alpha-nudiviruses.
A joint project of The University of Georgia - Warnell School of Forestry and Natural Resources and College of Agricultural and Environmental Sciences, The Center for Invasive Species and Ecosystem Health, USDA National Institute of Food and Agriculture, Southern Integrated Pest Management Center, Southern Plant Diagnostic Network, and USDA/APHIS Identification Technology Program, IPM Images image categories include: Commodity Groups; Taxonomy; Biological Controls; Damage Types; and Diseases.
What is the role of pathogens in Honey Bee Colony Collapse Disorder? Deep RNA sequencing was used to study microbial diversity and seek answers.